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| HAL : hal-00140614, version 1 |
| DOI : 10.1016/j.bios.2005.06.002 |
| Fiche détaillée |  Récupérer au format |
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| Biosensors & bioelectronics 21, 7 (2006) 1393-1402 |
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| Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy |
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| Yanxia X. Hou 1Saloua Helali 1 |
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| (15/01/2006) |
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| Rhodopsin, the G protein-coupled receptor (GPCR) which mediates the sense of vision, was prepared from calf eyes and used as receptor enriched membrane fraction. In this study it was immobilized onto gold electrode by two different techniques: Langmuir-Blodgett (LP) and a strategy based on a self-assembled multilayer. We demonstrated that Langmuir and LB films of rhodopsin are not stable. Thus, in this study a new protein multilayer was prepared on gold electrode by building up layer-by-layer a self-assembled multilayer. It is composed of a mixed self-assembled monolayer formed by MHDA and biotinyl-PE, followed by a biotin-avidin system which allows binding of biotinylated antibody specific to rhodopsin. The immobilization of rhodopsin in membrane fraction, by the specific antibody bound previously on self-assembled multilayer, was monitored with electrochemical impedance spectroscopy (EIS). In addition, the specificity and sensitivity of this self-assembled multilayer system to the presence of rhodopsin were investigated. No effect was observed when the system was in contact with olfactory receptor 17 in membrane fraction used for control measurements. All these results demonstrate that rhodopsin can be immobilized efficiently, specifically, quantitatively and stably on gold electrode through the self-assembled multilayer. |
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| 1 : | Centre de génie électrique de Lyon (CEGELY) |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| 2 : | Ampère |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| 3 : | Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA) |
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Institut national de la recherche agronomique (INRA) : UR1197 |
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| 4 : | Laboratory of NanoBioEngineering |
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Universitat de Barcelona |
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| 5 : | Sciences Analytiques (SA) |
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CNRS : UMR5180 – Université Claude Bernard - Lyon I |
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| Domaine | : | Sciences de l'ingénieur/Energie électrique |
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| rhodopsin – self-assembled multilayer – Langmuir-Blodgett – electrochemical impedance spectroscopy – cyclic voltammetry – biosensor – LANGMUIR-BLODGETT-FILMS – PROTEIN-COUPLED RECEPTORS – MONOLAYERS – IMMUNOSENSORS – BIOSENSORS – SURFACES – ANTIBODY – COMPLEX – SENSORS – ROUTES |
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| hal-00140614, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00140614 | |
| oai:hal.archives-ouvertes.fr:hal-00140614 | |
| Contributeur : Publications Ampère | |
| Soumis le : Samedi 7 Avril 2007, 12:44:25 | |
| Dernière modification le : Mercredi 18 Mars 2009, 15:36:53 | |
