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| HAL : hal-00140604, version 1 |
| DOI : 10.1016/j.electacta.2006.03.086 |
| Fiche détaillée |  Récupérer au format |
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| Electrochimica Acta 51, 24 (2006) 5182-5186 |
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| A disposable immunomagnetic electrochemical sensor based on functionalised magnetic beads on gold surface for the detection of atrazine |
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Saloua Helali 1Claude Martelet 1, 2 |
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| (15/07/2006) |
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| A disposable immunomagnetic electrochemical sensor involving magnetic particles was developed and applied for the detection of atrazine. The sensor was based on a magnetic monolayer of magnetic particles coated with streptavidin, formed on a gold electrode after application of a magnetic field. The magnetic monolayer was characterized using faradaic impedance spectroscopy, cyclic voltammetry and atomic force microscopy (AFM) techniques. The magnetic monolayer formation leads to an important change in constant phase element due to a change in thickness. AFM images show that the magnetic monolayer is formed and is very dense. The atrazine interacts with biotinyl-Fab fragment K47 and the immunoreaction was characterized by impedance spectroscopy. A decrease in electron transfer resistance was observed which could be attributed to rearrangements in the magnetic monolayer. This approach leads to a sensitive detection of atrazine, acting as an antigen, with a good linear response in the range 10-600 ng/ml. |
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| 1 : | Centre de génie électrique de Lyon (CEGELY) |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| 2 : | Ampère |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| Domaine | : | Sciences de l'ingénieur/Autre Chimie/Autre Sciences de l'ingénieur/Energie électrique |
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| atrazine – magnetic particles coated with streptavidin – impedance spectroscopy – AFM – biosensors – IMPEDANCE SPECTROSCOPY – IMMUNOSENSORS – BIOSENSORS – ELECTRODE – ANTIBODY |
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| hal-00140604, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00140604 | |
| oai:hal.archives-ouvertes.fr:hal-00140604 | |
| Contributeur : Publications Ampère | |
| Soumis le : Samedi 7 Avril 2007, 12:12:52 | |
| Dernière modification le : Jeudi 21 Février 2008, 15:16:32 | |
