%0 Journal Article
%T Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging
%+ Laboratoire Charles Coulomb (L2C)
%+ Mécanismes moléculaires dans les démences neurodégénératives (MMDN)
%A Manesco, Clara
%A Saavedra-Villanueva, Oscar
%A Martin, Marta
%A de Lizaraga, Joshua
%A Varga, Béla
%A Cloitre, Thierry
%A Gerber, Yannick Nicolas
%A Perrin, Florence Evelyne
%A Gergely, Csilla
%< avec comité de lecture
%@ 1549-9634
%J Nanomedicine: Nanotechnology, Biology and Medicine
%I Elsevier
%N 53
%P 102699
%8 2023-08-11
%D 2023
%R 10.1016/j.nano.2023.102699
%K Spinal cord injury (SCI)
%K Collagen Second harmonic generation (SHG)
%K Atomic force microscopy (AFM)
%K Nano-mechanics
%Z Life Sciences [q-bio]Journal articles
%X Spinal cord injury is a dramatic disease leading to severe motor, sensitive and autonomic impairments. After injury the axonal regeneration is partly inhibited by the glial scar, acting as a physical and chemical barrier. The scarring process involves microglia, astrocytes and extracellular matrix components, such as collagen, con- structing the fibrotic component of the scar. To investigate the role of collagen, we used a multimodal label-free imaging approach combining multiphoton and atomic force microscopy. The second harmonic generation signal exhibited by fibrillar collagen enabled to specifically monitor it as a biomarker of the lesion. An increase in collagen density and the formation of more tortuous fibers over time after injury are observed. Nano-mechanical investigations revealed a noticeable hardening of the injured area, correlated with collagen fibers' formation. These observations indicate the concomitance of important structural and mechanical modifications during the fibrotic scar evolution.
%G English
%L hal-04195286
%U https://hal.science/hal-04195286
%~ EPHE
%~ CNRS
%~ MMDN
%~ L2C
%~ PSL
%~ UNIV-MONTPELLIER
%~ TEST-DEV
%~ EPHE-PSL
%~ UM-2015-2021
%~ UM-EPE