%0 Journal Article
%T Non-toxic Polymeric Dots with the Strong Protein-Driven Enhancement of One- and Two-Photon Excited Emission for Sensitive and Non-destructive Albumin Sensing
%+ Laboratoire Charles Coulomb (L2C)
%+ Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
%+ Wroclaw University of Science and Technology
%A Mucha, Sebastian, Grzegorz
%A Piksa, Marta
%A Firlej, Lucyna
%A Krystyniak, Agnieszka
%A Różycka, Mirosława
%A Kazana, Wioletta
%A Pawlik, Krzysztof
%A Samoć, Marek
%A Matczyszyn, Katarzyna
%< avec comité de lecture
%@ 1944-8244
%J ACS Applied Materials & Interfaces
%I Washington, D.C. : American Chemical Society
%V 14
%N 35
%P 40200-40213
%8 2022
%D 2022
%R 10.1021/acsami.2c08858
%K polymer dots
%K non-toxic nanomaterials
%K serum albumins
%K two-photon excited fluorescence
%K fluorescence enhancement
%K albumin's probe
%K nanomarkers
%K biosensing
%Z Chemical Sciences/Polymers
%Z Chemical Sciences/Analytical chemistry
%Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]Journal articles
%X The need for efficient probing, sensing, and control of the bioactivity of biomolecules (e.g., albumins) has led to the engineering of new fluorescent albumins' markers fulfilling very specific chemical, physical, and biological requirements. Here, we explore acetone-derived polymer dots (PDs) as promising candidates for albumin probes, with special attention paid to their cytocompatibility, two-photon absorption properties, and strong ability to non-destructively interact with serum albumins. The PDs show no cytotoxicity and exhibit high photostability. Their pronounced green fluorescence is observed upon both onephoton excitation (OPE) and two-photon excitation (TPE). Our studies show that both OPE and TPE emission responses of PDs are proteinaceous environment-sensitive. The proteins appear to constitute a matrix for the dispersion of fluorescent PDs, limiting both their aggregation and interactions with the aqueous environment. It results in a large enhancement of PD fluorescence. Meanwhile, the PDs do not interfere with the secondary protein structures of albumins, nor do they induce their aggregation, enabling the PD candidates to be good nanomarkers for non-destructive probing and sensing of albumins.
%G English
%2 https://hal.science/hal-03772070/document
%2 https://hal.science/hal-03772070/file/acsami.2c08858.pdf
%L hal-03772070
%U https://hal.science/hal-03772070
%~ CNRS
%~ L2C
%~ UNIV-MONTPELLIER
%~ UM-2015-2021
%~ UM-EPE