%0 Journal Article
%T Mechanical instabilities of aorta drive blood stem cell production: a live study
%+ Unité de Recherches Zootechniques (URZ)
%+ Laboratoire Charles Coulomb (L2C)
%+ Laboratory of Pathogen Host Interactions [Montpellier] (LPHI)
%+ Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP)
%+ Southern Federal University [Rostov-on-Don] (SFEDU)
%+ Dynamique des interactions membranaires normales et pathologiques (DIMNP)
%A Poullet, Nausicaa
%A Golushko, Ivan
%A Lorman, Vladimir
%A Travnickova, Jana
%A Bureau, Charlotte
%A Chalin, Dmitrii
%A Rochal, Sergei
%A Parmeggiani, Andrea
%A Kissa, Karima
%Z NOTICE A REPRENDRE PAS DE CLE UT AU 15/07
%< avec comité de lecture
%@ 1420-682X
%J Cellular and Molecular Life Sciences
%I Springer Verlag
%V 77
%N 17
%P 3453-3464
%8 2020-09
%D 2020
%R 10.1007/s00018-019-03372-2
%M 31732791
%K modeling
%K 4D microscopy
%K endothelial-to-haematopoietic transition
%K haematopoeiesis
%K zebrafish
%Z Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
%Z Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]Journal articles
%X During embryogenesis of all vertebrates, haematopoietic stem/progenitor cells (HSPCs) extrude from the aorta by a complex process named endothelial-to-haematopoietic transition (EHT). HSPCs will then colonize haematopoietic organs allowing haematopoiesis throughout adult life. The mechanism underlying EHT including the role of each aortic endothelial cell (EC) within the global aorta dynamics remains unknown. In the present study, we show for the first time that EHT involves the remodelling of individual cells within a collective migration of ECs which is tightly orchestrated, resulting in HSPCs extrusion in the sub-aortic space without compromising aorta integrity. By performing a cross-disciplinary study which combines high-resolution 4D imaging and theoretical analysis based on the concepts of classical mechanics, we propose that this complex developmental process is dependent on mechanical instabilities of the aorta preparing and facilitating the extrusion of HSPCs.
%G English
%L hal-03291847
%U https://hal.inrae.fr/hal-03291847
%~ CIRAD
%~ CNRS
%~ UNIV-MONTP1
%~ UNIV-MONTP2
%~ DIMNP
%~ L2C
%~ AGREENIUM
%~ MIPS
%~ BA
%~ BS
%~ UNIV-MONTPELLIER
%~ LPHI
%~ INSTITUT-AGRO-MONTPELLIER
%~ INRAE
%~ INRAEOCCITANIEMONTPELLIER
%~ GENETIQUE_ANIMALE
%~ ANR
%~ AGAP
%~ ASSET
%~ UM-2015-2021
%~ RESEAU-EAU
%~ INSTITUT-AGRO