%0 Conference Paper
%F Oral 
%T Physical modeling of the ParBS complex: insight into Liquid-Liquid Phase Separation
%+ Laboratoire Charles Coulomb (L2C)
%A Walter, Jean-Charles
%F Invité
%< sans comité de lecture
%Z L2C:21-035
%B BioPhyChrom 2021 (The biology and physics of bacterial chromosome organization)
%C Leiden, Netherlands
%8 2021-04-29
%D 2021
%Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Conference papers
%X The ParABS system is composed of three components: a centromeric sequence, parS, and two proteins, the ParA (ATPase) and the ParB DNA binding proteins. Hundreds of ParB proteins assemble dynamically to form nucleoprotein parS-anchored complexes (ParBS) that serve as substrates for ParA molecules to catalyze positioning and segregation events. We address the theoretical modeling of ParBS with a Lattice Gas models and compare it to experiments [1]. We give evidences that (i) ParBS is formed via a Liquid-Liquid Phase Separation in the metastable region of the phase diagram and (ii) this nucleation is catalyzed with the parS sequence [1].[1] Guilhas, Walter,... & Nollmann (2020). ATP-driven separation of liquid phase condensates in bacteria. Mol. Cell, 79, 293-303.
%G English
%L hal-03215528
%U https://hal.science/hal-03215528
%~ CNRS
%~ L2C
%~ MIPS
%~ UNIV-MONTPELLIER
%~ UM-2015-2021