%0 Journal Article
%T Self-Assembling Peptide—Polymer Nano-Objects via Polymerization-Induced Self-Assembly
%+ Institut Européen des membranes (IEM)
%+ Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM)
%+ Laboratoire Charles Coulomb (L2C)
%+ Laboratoire de Chimie des Polymères Organiques (LCPO)
%+ Team 3 LCPO : Polymer Self-Assembly & Life Sciences
%+ Eindhoven University of Technology [Eindhoven] (TU/e)
%+ Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM)
%A Dao, Tuyen, T. P.
%A Vezenkov, Lubomir
%A Subra, Gilles
%A Amblard, Muriel
%A In, Martin
%A Le Meins, Jean-François
%A Aubrit, Florian
%A Moradi, Mohammad-Amin
%A Ladmiral, Vincent
%A Semsarilar, M.
%Z CNRS “Osez l’interdisciplinarité” 2017
%< avec comité de lecture
%@ 0024-9297
%J Macromolecules
%I American Chemical Society
%V 53
%N 16
%P 7034-7043
%8 2020-08-11
%D 2020
%R 10.1021/acs.macromol.0c01260
%K RAFT polymerization
%K Peptides and proteins
%K Copolymerization
%K Copolymers
%K Transmission electron microscopy TEM
%K Small angle X-ray scattering SAXS
%K Depolarized dynamic light scattering DDLS
%Z Chemical Sciences/Polymers
%Z Chemical Sciences/Material chemistry
%Z Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]Journal articles
%X Self-assembling peptides (SAPs) have been extensively studied for their ability to form nanoscale ordered structures driven by noncovalent molecular interactions. Meanwhile, polymerization-induced self-assembly (PISA) has been exploited as a facile and efficient way to produce various amphiphilic block copolymer nano-objects, whose self-assembly was governed predominantly by the interactions of the different blocks with the polymerization medium. In this work, we combined PISA with SAPs to prepare novel peptide–polymer hybrid nano-objects, thus harnessing the advantages of PISA and the self-assembling driving force of SAPs. A tripeptide methacrylamide derivative (MAm-Gly-Phe-Phe-NH2, denoted as MAm-GFF, where MAm means methacrylamide) was copolymerized with glycerol monomethacrylate (GMA) to produce a P(GMA65-stat-(MAm-GFF)7) macro-chain transfer agent (macro-CTA) by reversible addition–fragmentation chain transfer polymerization in dimethylformamide. This peptide-based macro-CTA was then successfully chain-extended with poly(2-hydroxypropyl methacrylate) (PHPMA) by aqueous dispersion PISA, forming P(GMA65-stat-(MAm-GFF)7)-b-PHPMA28 self-assembled objects. Fibrous structures were observed by transmission electron microscopy (TEM) and Cryo-TEM, in agreement with depolarized dynamic light scattering, static light scattering, and small-angle X-ray scattering experiments that also revealed long anisotropic morphologies. Such structures have not been reported previously for PISA-prepared nano-objects. This confirms the decisive influence of the GFF SAP on the self-assembly. In addition, annealing the PISA suspension at different temperatures led to a significant size decrease in the self-assembled objects and to a morphological transition caused by the thermosensitivity of both the core-forming PHPMA block and the stabilizing P(GMA-stat-(MAm-GFF)) block.
%G English
%2 https://hal.science/hal-02915614/document
%2 https://hal.science/hal-02915614/file/revised%20draft-%20Macromolecule.pdf
%L hal-02915614
%U https://hal.science/hal-02915614
%~ CNRS
%~ ENSC-MONTPELLIER
%~ ICG
%~ IBMM
%~ IEM
%~ OPENAIRE
%~ L2C
%~ INC-CNRS
%~ MIPS
%~ CHIMIE
%~ UNIV-MONTPELLIER
%~ TEST-HALCNRS
%~ LCPO
%~ ANR
%~ UM-2015-2021
%~ TEST2-HALCNRS