Electrochemical strategies for gallic acid detection: Potential for application in clinical, food or environmental analyses

Mihaela Badea (1) , Federico Di Modugno (2) , Laura Floroian (3) , Delia Mirela Tit (4) , Patrizia Restani (2) , Simona Bungau (4) , Ciprian Iovan (4) , Gabriela Elena Badea (4) , Lotfi Aleya (5)

1 Department of Fundamental, Prophylactic and Clinical Disciplines, Faculty of Medicine, Transilvania University of Brasov, Brasov, Romania
2 UNIMI - Università degli Studi di Milano = University of Milan
3 Department of Automation and Information Technology, Faculty of Electrical Engineering and Computer Sciences, Transylvania University of Brasov, Brasov, Romania
4 University of Oradea
5 LCE - Laboratoire Chrono-environnement (UMR 6249)

Polyphenols are important to human health thus making it interesting and necessary to identify and assess methods for their detection. Gallic acid (GA) is a well-known antioxidant compound, found in tea leaves, various fruits, fruit seeds and in fruit-derived foods and beverages. In this study, to electrochemically detect this compound and assess the potential for GA detection, different analytical conditions at pH values of 5.8, 7 and 8 were tried. Two types of device were used for GA detection: (1) Lazar ORP-146C reduction-oxidation microsensors, coupled with a Jenco device, for estimation of antioxidant capacities of different electroactive media, and (2) screen-printed carbon sensors coupled with a mobile PalmSens device using differential pulse voltammetry (qualitative and quantitative GA determination). These proposed methods were validated by analysing some real samples: wine, green tea, apple juice and serum fortified with GA. Detection was evaluated in terms of specific calibration curves, with low limit of detection (LOD) and limit of quantification (LOQ), low response time, and high sensitivities. The analytical characteristics obtained recommend these methods to be tested on more other types of real samples. Our proposed methods, used in the established conditions of pH, may have further application in other clinical, food or environmental samples analyses in which the results of total antioxidants contents are usually expressed in GA equivalents.

Mots clés: Electrochemical detection; Gallic acid; Differential pulse voltammetry

Domaines: Sciences de l'environnement

Mihaela Badea, Federico Di Modugno, Laura Floroian, Delia Mirela Tit, Patrizia Restani, et al.. Electrochemical strategies for gallic acid detection: Potential for application in clinical, food or environmental analyses. Science of the Total Environment, 2019, 672, pp.129-140. ⟨10.1016/j.scitotenv.2019.03.404⟩. ⟨hal-02092991⟩