%0 Conference Paper
%F Oral 
%T DNA-loops and the organization of protein-DNA complexes in bacteria
%+ Laboratoire Charles Coulomb (L2C)
%+ Systèmes Complexes et Phénomènes Nonlinéaires (SCPN)
%A Walliser, Nils-Ole
%< sans comité de lecture
%Z L2C:18-341
%B Journées de Physique Statistique 2018
%C Paris, France
%8 2018-01-25
%D 2018
%Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Conference papers
%X The DNA shows a high degree of spatial and dynamical organization over a broad range of length scales. It interacts with different populations of proteins and can form protein-DNA complexes that underlie various biological processes, including chromosome segregation. A prominent example is the large ParB-DNA complex, an essential component of a widely spread mechanism for DNA segregation in bacteria. Recent studies suggest that DNA-bound ParB proteins interact with each other and condense into large clusters with multiple extruding DNA-loops. In my talk, I present the Looping and Clustering model, a simple statistical physics approach to describe how proteins assemble into a protein-DNA cluster with multiple loops. Our analytic model predicts binding profiles of ParB proteins in good agreement with data from high precision ChIP-sequencing – a biochemical technique to analyze the interaction between DNA and proteins at the level of the genome. The Looping and Clustering framework provides a quantitative tool that could be exploited to interpret further experimental results of ParB-like protein complexes and gain some new insights into the organization of DNA.
%G English
%L hal-01939889
%U https://hal.science/hal-01939889
%~ CNRS
%~ L2C
%~ MIPS
%~ UNIV-MONTPELLIER
%~ UM-2015-2021