Detection of carbapenemase activity in Pseudomonas aeruginosa by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Guillaume Miltgen (1, 2) , P. Plésiat (3, 4, 5) , A. Mille (4) , P. Chatelain (4) , D. Fournier (4, 3, 5)

1 Service de bactériologie, parasitologie, virologie et hygiène hospitalière [La réunion]
2 PIMIT - Processus Infectieux en Milieu Insulaire Tropical
3 Service de bactériologie [Besançon]
4 CNR - Centre National de Référence de la Résistance aux Antibiotiques
5 LCE - Laboratoire Chrono-environnement (UMR 6249)

A new MALDI-TOF MS imipenem hydrolysis assay was developed to optimize detection of carbapenemase-producing strains of Pseudomonas aeruginosa. The method correctly discriminated carbapenemase producers (n=74) from non-producers (n=95) in 99.4% of cases, and successfully detected 100% GES-5 strains (n=5).

Mots clés: Carbapenemase; Detection; MALDI-TOF MS; Pseudomonas aeruginosa

Domaines: Bactériologie

Guillaume Miltgen, P. Plésiat, A. Mille, P. Chatelain, D. Fournier. Detection of carbapenemase activity in Pseudomonas aeruginosa by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Journal of Microbiological Methods, 2018, 145, pp.66 - 68. ⟨10.1016/j.mimet.2017.12.011⟩. ⟨hal-01684649⟩