%0 Journal Article %T Characterization of recombinant Trypanosoma brucei gambiense Translationally Controlled Tumor Protein (r Tbg TCTP) and its interaction with Glossina midgut bacteria %+ Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP) %+ Institut méditerranéen d'océanologie (MIO) %+ Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) %+ Interaction hôtes vecteurs parasites dans les infections par des trypanosomatidae (UMR InterTryp ) %A Bossard, Géraldine %A Bartoli, Manon %A Fardeau, Marie-Laure %A Holzmuller, Philippe, P. %A Ollivier, Bernard %A Geiger, Anne %Z IRD (UMR INTERTRYP); CIRAD %< avec comité de lecture %@ 1949-0976 %J Gut microbes %I Taylor & Francis %V 8 %N 5 %P 413 - 427 %8 2017-05-25 %D 2017 %R 10.1080/19490976.2017.1331833 %M 28586253 %K bacteria midgut flora %K Translationally Controlled Tumor Protein (TCTP) %K Glossina %K Trypanosoma brucei gambiense %Z Life Sciences [q-bio]/Ecology, environmentJournal articles %X In humans, sleeping sickness (i.e. Human African Trypanosomiasis) is caused by the protozoan parasites Trypanosoma brucei gambiense (Tbg) in West and Central Africa, and T. b. rhodesiense in East Africa. We previously showed in vitro that Tbg is able to excrete/secrete a large number of proteins, including Translationally Controlled Tumor Protein (TCTP). Moreover, the tctp gene was described previously to be expressed in Tbg-infected flies. Aside from its involvement in diverse cellular processes, we have investigated a possible alternative role within the interactions occurring between the trypanosome parasite, its tsetse fly vector, and the associated midgut bacteria. In this context, the Tbg tctp gene was synthesized and cloned into the baculovirus vector pAcGHLT-A, and the corresponding protein was produced using the baculovirus Spodoptera frugicola (strain 9) / insect cell system. The purified recombinant protein rTbgTCTP was incubated together with bacteria isolated from the gut of tsetse flies, and was shown to bind to 24 out of the 39 tested bacteria strains belonging to several genera. Furthermore, it was shown to affect the growth of the majority of these bacteria, especially when cultivated under microaerobiosis and anaerobiosis. Finally, we discuss the potential for TC IP to modulate the fly microbiome composition toward favoring trypanosome survival. %G English %2 https://amu.hal.science/hal-01621726/document %2 https://amu.hal.science/hal-01621726/file/bossard_gutmicrobes_2018_1.pdf %L hal-01621726 %U https://amu.hal.science/hal-01621726 %~ IRD %~ CIRAD %~ INSU %~ UNIV-TLN %~ CNRS %~ UNIV-AMU %~ UNIV-LYON1 %~ INRA %~ MIO %~ OSU-INSTITUT-PYTHEAS %~ GIP-BE %~ AGROPOLIS %~ AGREENIUM %~ UDL %~ UNIV-LYON %~ INRAE %~ INRAEOCCITANIEMONTPELLIER %~ MIO-MEB %~ FRM