%0 Journal Article
%T Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist.
%+ Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM)
%+ Centre National de la Recherche Scientifique (CNRS)
%+ Université de Montpellier (UM)
%+ Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)
%+ Abeilles et Environnement (AE)
%+ Évolution, génomes, comportement et écologie (EGCE)
%+ Université Paris-Sud - Paris 11 (UP11)
%+ Institut de Recherche pour le Développement (IRD [France-Ouest])
%+ Université Paris-Saclay
%+ Signalisation Fonctionnelle des Canaux Ioniques et Récepteurs (SIFCIR)
%+ Centre de Recherches sur la Cognition Animale - UMR5169 (CRCA)
%+ Université Toulouse III - Paul Sabatier (UT3)
%+ Centre de recherche de l’Institut universitaire en santé mentale de Québec [Canada] (CERVO)
%A Rousset, Matthieu
%A Collet, Claude
%A Cens, Thierry
%A Bastin, Florian
%A Raymond, Valérie
%A Massou, Isabelle
%A Menard, Claudine
%A Thibaud, Jean-Baptiste
%A Charreton, Mercedes
%A Vignes, Michel
%A Chahine, Mohamed
%A Sandoz, Jean-Christophe
%A Charnet, Pierre
%Z ANR Bee- Channels N° ANR-13-BSV7-0010-0
%< avec comité de lecture
%@ 2045-2322
%J Scientific Reports
%I Nature Publishing Group
%V 7
%P 41782
%8 2017
%D 2017
%R 10.1038/srep41782
%M 28145504
%K myocyte
%K bee
%K in situ hybridization
%K activation cellulaire
%K xenopus oocyte
%K hybridation in situ
%K canal calcique
%K apis mellifera
%K neurone récepteur
%K abeille
%Z Life Sciences [q-bio]Journal articles
%X Voltage-gated Ca(2+) channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca(2+) channel genes, grouped in 3 families (CaV1, CaV2 and CaV3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca(2+) channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type CaV3 Ca(2+) channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca(2+) currents recorded in bee neurons and myocytes with Ca(2+) currents recorded in Xenopus oocytes expressing the honeybee CaV3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca(2+) channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a CaV3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of CaV3 suggest a role in honeybee muscle function.
%G English
%2 https://hal.science/hal-01595255/document
%2 https://hal.science/hal-01595255/file/Honey._%7B9F01FB34-35C6-4299-A69F-4CEBAD5AF169%7D.pdf
%L hal-01595255
%U https://hal.science/hal-01595255
%~ IRD
%~ UNIV-AVIGNON
%~ UNIV-TLSE2
%~ UNIV-TLSE3
%~ CNRS
%~ UNIV-ANGERS
%~ UNIV-PSUD
%~ ENSC-MONTPELLIER
%~ INRA
%~ IBMM
%~ CRCA
%~ INC-CNRS
%~ UNIV-PARIS-SACLAY
%~ AGREENIUM
%~ UNIV-PSUD-SACLAY
%~ CHIMIE
%~ UNIV-MONTPELLIER
%~ SIFCIR
%~ INRAE
%~ TEST-HALCNRS
%~ EGCE
%~ AE
%~ UNIV-UT3
%~ UT3-INP
%~ UT3-TOULOUSEINP
%~ UM-2015-2021
%~ TEST3-HALCNRS
%~ TEST4-HALCNRS
%~ INRAEPACA
%~ TEST5-HALCNRS