%0 Conference Proceedings
%T Microbiology of shallow subsurface aquifer and carbonate rocks studied by culture-dependent and culture-independent methods. Preliminary results on an underground laboratory, the LSBB, Rustrel, France
%+ Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE)
%+ Institut méditerranéen d'océanologie (MIO)
%+ Biodiversité et Biotechnologie Fongiques (BBF)
%+ Laboratoire Souterrain à Bas Bruit (LSBB)
%+ Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH)
%A Gales, Gregoire
%A Erauso, Gaël
%A Cayol, Jean-Luc
%A Navarro, David
%A Boyer, Daniel
%A Micolau, Gilles
%< avec comité de lecture
%( I-DUST 2016 - INTER-DISCIPLINARY UNDERGROUND SCIENCE & TECHNOLOGY
%B 6. International Conference on Inter-Disciplinary Underground Science and Technology (-DUST)
%C Avignon, France
%I EDP Sciences
%C Les Ulis (france)
%3 E3S Web of Conferences
%V 12
%P 5
%8 2016-06-01
%D 2016
%R 10.1051/e3sconf/20161205002
%K groundwater table
%K carbonate rock
%K anthropic activity
%K laboratory study
%K étude en laboratoire
%K culture anaérobie
%K activité anthropique
%K procédé aérobie
%K microbiologie du sol
%K nappe souterraine
%K aquifère
%K roche carbonatee
%Z Engineering Sciences [physics]/OtherConference papers
%X We investigated the microbiology of a shallow subsurface site, the LSBB, located near Avignon. This site lies in carbonate rocks, belonging to the Urgonian facies. Rock, concrete and water samples were collected and directly transferred to the laboratory. Studies of microorganisms as pure cultures are the only way to get their real physiological properties. Nevertheless, microbiologists cannot cultivate and isolate the majority of microorganisms for several reasons, one being our lack of understanding of their minimal needs. Molecular studies, e.g. extraction and sequencing of the total nucleic acids present in an environment provide phylogenetic and metabolic information on uncultivated microorganisms. We performed aerobic and anaerobic culture with various electron acceptors and donors, searching for heterotrophic, methanogenic, sulphate-nitrate-and FeIII-reducing Prokaryotes. We also performed DNA extractions and PCR amplification of ribosomal RNA genes, to test if our protocols were adapted to this environment. Our results show that the LSBB galleries are colonized by a low diversity microbiote, with a strong influence of anthropogenic activities. Further studies will link the microorganisms biodiversity and the petrophysic properties of rocks.
%G English
%L hal-01594526
%U https://hal.science/hal-01594526
%~ INSU
%~ UNICE
%~ UNIV-AVIGNON
%~ UNIV-TLN
%~ CNRS
%~ UNIV-AMU
%~ CDF
%~ INRA
%~ EC-MARSEILLE
%~ OCA
%~ MIO
%~ CEREGE
%~ OSU-INSTITUT-PYTHEAS
%~ PSL
%~ AGREENIUM
%~ UNIV-COTEDAZUR
%~ INRAE
%~ CDF-PSL
%~ MIO-MEB
%~ EMMAH
%~ LSBB
%~ EMMAH-EQUIPE-AVIGNON
%~ INRAEPACA