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Article Dans Une Revue American Journal of Industrial Medicine Année : 2016

Hypersensitivity pneumonitis in a beautician.

Résumé

A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and adenosine-5'-triphosphate-3'-diphosphate (pppApp) has been developed. The assay method is based on competition of an unlabeled highly phosphorylated nucleotide with 3H-labeled highly phosphorylated nucleotide for binding sites on a specific antibody. Antibodies to ppGpp and pppApp were obtained by immunizing rabbits with the antigen prepared by conjugating ppGpp with human serum albumin using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, and with the antigen prepared by conjugating 8-(6-aminohexyl)amino-adenosine-5'-triphosphate-3'-diphosphate with human serum albumin using glutaraldehyde, respectively. Antibody-bound 3H-labeled highly phosphorylated nucleotides were separated from the free 3H-labeled highly phosphorylated nucleotides by selective adsorption on dextran-coated charcoal. Displacement plots were linear over a concentration range of 5-1,000 pmol/assay tube in a log-probit percentage plot. Application of this method to biological systems offers improved accuracy and convenience compared with the previous 32PO4-labeling technique.

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Dates et versions

hal-01368327 , version 1 (19-09-2016)

Identifiants

  • HAL Id : hal-01368327 , version 1
  • PUBMED : 27427424

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Thibaud Soumagne, Gabriel Reboux, Bruno Degano, Jean Charles Dalphin. Hypersensitivity pneumonitis in a beautician.. American Journal of Industrial Medicine, 2016, 88 (6), pp.1785-92. ⟨hal-01368327⟩
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