%0 Book Section %T Methods for Studying Microorganisms in the Environment %+ Laboratoire d'Océanographie Microbienne (LOMIC) %+ Institut méditerranéen d'océanologie (MIO) %+ Ecologie des systèmes marins côtiers (Ecosym) %+ Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE) %+ Observatoire océanologique de Banyuls (OOB) %+ Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) %+ Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) %+ Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM) %+ Institut pluridisciplinaire de recherche sur l'environnement et les matériaux (IPREM) %A Joux, Fabien %A Bertrand, Jean Claude %A de Wit, Rutger %A Grossi, Vincent %A Intertaglia, Laurent %A Lebaron, Philippe %A Michotey, Valerie %A Normand, Philippe %A Peyret, Pierre, P. %A Raimbault, Patrick %A Tamburini, Christian %A Urios, Laurent %@ 978-94-017-9117-5 %B Environmental Microbiology: Fundamentals and Applications %I Springer Netherlands %P 757-829 %8 2014-07-17 %D 2014 %R 10.1007/978-94-017-9118-2_17 %K Bacterial isolation %K Biomarkers %K Cultural techniques %K Cytometry %K DNA microarray %K Microbial activities %K Microelectrodes %K Molecular fingerprints %K PCR %K Phospholipid fatty acid %K analyses %K Pigment analyses %K Sampling techniques %Z Life Sciences [q-bio]/Ecology, environmentBook sections %X The main methods for the study of microorganisms in the environment (water, soil, sediment, biofilms), the different techniques of sampling for measuring biomass, the activities, and the diversity of the microorganisms are presented. To respond to these various issues, techniques as varied as those of flow cytometry, molecular biology, biochemistry, molecular isotopic tools, or electrochemistry are implemented. These different techniques are described with their advantages and disadvantages for different types of biotopes. The question of the isolation, culture, and conservation of microorganisms from the environment are also addressed. Without being exhaustive, this chapter emphasizes the importance of using appropriate and efficient methodological tools to properly explore the still mysterious compartment of microorganisms in the environment. %G English %L hal-01235538 %U https://hal.sorbonne-universite.fr/hal-01235538 %~ UNIV-ST-ETIENNE %~ ENS-LYON %~ INSU %~ UPMC %~ UNIV-TLN %~ PRES_CLERMONT %~ CNRS %~ UNIV-AMU %~ UNIV-PAU %~ UNIV-CLERMONT1 %~ UNIV-LYON1 %~ UNIV-MONTP1 %~ UNIV-MONTP2 %~ IFREMER %~ INRA %~ LGL-TPE %~ MIO %~ OSU-INSTITUT-PYTHEAS %~ GIP-BE %~ IPREM %~ LOMIC %~ LBBM %~ CIDAM %~ BIOENVIS %~ AGROPOLIS %~ AGREENIUM %~ UPMC_POLE_3 %~ EDF %~ UNIV-MONTPELLIER %~ MEDIS %~ SORBONNE-UNIVERSITE %~ SU-SCIENCES %~ SU-SCI %~ UMS-2348 %~ UDL %~ UNIV-LYON %~ INRAE %~ MIO-CEM %~ MIO-MEB %~ ECOMIC %~ ALLIANCE-SU %~ UM1-UM2