%0 Journal Article
%T Welcome to pandoraviruses at the 'Fourth TRUC' club
%+ Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE)
%+ Institut de Mathématiques de Marseille (I2M)
%+ Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille)
%+ Medical Parasitology Laboratory
%A Sharma, Vikas
%A Colson, Philippe
%A Chabrol, Olivier
%A Scheid, Patrick
%A Pontarotti, Pierre
%A Raoult, Didier
%< avec comité de lecture
%@ 1664-302X
%J Frontiers in Microbiology
%I Frontiers Media
%V 6
%N 423
%8 2015-05-18
%D 2015
%R 10.3389/fmicb.2015.00423
%K giant virus
%K Pandoravirus
%K nucleocytoplasmic large DNA viruses
%K Megavirales
%K informational genes
%K domains of life
%K TRUC
%K phylogeny
%K DNA VIRUSES
%K GENOME SEQUENCE
%K GIANT VIRUSES
%K 1ST ISOLATION
%K MIMIVIRUS
%K MARSEILLEVIRUS
%K HIGHLIGHTS
%K EVOLUTION
%K AMEBAS
%K ORIGIN
%Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles
%X Nucleocytoplasmic large DNA viruses, or representatives of the proposed order Megavirales, belong to families of giant viruses that infect a broad range of eukaryotic hosts. Megaviruses have been previously described to comprise a fourth monophylogenetic TRUC (things resisting uncompleted classification) together with cellular domains in the universal tree of life. Recently described pandoraviruses have large (1.9-2.5 MB) and highly divergent genomes. In the present study, we updated the classification of pandoraviruses and other reported giant viruses. Phylogenetic trees were constructed based on six informational genes. Hierarchical clustering was performed based on a set of informational genes from Megavirales members and cellular organisms. Homologous sequences were selected from cellular organisms using TimeTree software, comprising comprehensive, and representative sets of members from Bacteria, Archaea, and Eukarya. Phylogenetic analyses based on three conserved core genes clustered pandoraviruses with phycodnaviruses, exhibiting their close relatedness. Additionally, hierarchical clustering analyses based on informational genes grouped pandoraviruses with Megavirales members as a super group distinct from cellular organisms. Thus, the analyses based on core conserved genes revealed that pandoraviruses are new genuine members of the 'Fourth TRUC' club, encompassing distinct life forms compared with cellular organisms.
%G English
%2 https://amu.hal.science/hal-01211489/document
%2 https://amu.hal.science/hal-01211489/file/ptpmcrender.pdf
%L hal-01211489
%U https://amu.hal.science/hal-01211489
%~ INSERM
%~ IRD
%~ CNRS
%~ UNIV-AMU
%~ EC-MARSEILLE
%~ I2M
%~ I2M-2014-