%0 Journal Article
%T Stochastic Self-Assembly of ParB Proteins Builds the Bacterial DNA Segregation Apparatus
%+ Laboratoire de microbiologie et génétique moléculaires - UMR5100 (LMGM)
%+ Centre de Biochimie Structurale [Montpellier] (CBS)
%+ Laboratoire Charles Coulomb (L2C)
%A Sanchez, Aurore
%A Cattoni, Diego
%A Walter, Jean-Charles
%A Rech, Jérôme
%A Parmeggiani, Andrea
%A Nollmann, Marcelo
%A Bouet, Jean-Yves
%< avec comité de lecture
%Z L2C:15-131
%@ 2405-4712
%J Cell Systems
%I Elsevier
%V 1
%P 163-173
%8 2015-08-26
%D 2015
%R 10.1016/j.cels.2015.07.013
%Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
%Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
%Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/BiophysicsJournal articles
%X Many canonical processes in molecular biology rely on the dynamic assembly of higher-order nucleoprotein complexes. In bacteria, the assembly mechanism of ParABS, the nucleoprotein super-complex that actively segregates the bacterial chromosome and many plasmids, remains elusive. We combined super-resolution microscopy, quantitative genome-wide surveys, biochemistry, and mathematical modeling to investigate the assembly of ParB at the centromere-like sequences parS. We found that nearly all ParB molecules are actively confined around parS by a network of synergistic protein-protein and protein-DNA interactions. Interrogation of the empirically determined, high-resolution ParB genomic distribution with modeling suggests that instead of binding only to specific sequences and subsequently spreading, ParB binds stochastically around parS over long distances. We propose a new model for the formation of the ParABS partition complex based on nucleation and caging: ParB forms a dynamic lattice with the DNA around parS.This assembly model and approach to characterizing large-scale, dynamic interactions between macro-molecules may be generalizable to many unrelated machineries that self-assemble in superstructures.
%G English
%L hal-01191677
%U https://hal.science/hal-01191677
%~ UNIV-TLSE3
%~ CNRS
%~ CBS
%~ L2C
%~ MIPS
%~ BS
%~ UNIV-MONTPELLIER
%~ ANR
%~ UNIV-UT3
%~ UT3-INP
%~ UT3-TOULOUSEINP
%~ UM-2015-2021
%~ LMGM
%~ FRM