%0 Journal Article
%T Aminobacterium thunnarium sp. nov., a mesophilic, amino acid-degrading bacterium isolated from an anaerobic sludge digester, pertaining to the phylum Synergistetes.
%+ Institut méditerranéen d'océanologie (MIO)
%+ Laboratoire d'Ecologie et de Technologie Microbiennes (LETMi)
%A Hamdi, Olfa
%A Ben Hania, Wajdi
%A Postec, Anne
%A Bouallagui, Hassib
%A Hamdi, Moktar
%A Bonin, Patricia
%A Ollivier, Bernard
%A Fardeau, Marie-Laure
%< avec comité de lecture
%Z MIO:15-032
%@ 1466-5026
%J International Journal of Systematic and Evolutionary Microbiology
%I Microbiology Society
%V 65
%N Pt 2
%P 609-14
%8 2015-02
%D 2015
%R 10.1099/ijs.0.068965-0
%M 25406236
%Z Sciences of the Universe [physics]/Ocean, Atmosphere
%Z Environmental Sciences/Biodiversity and EcologyJournal articles
%X A new Gram-staining-positive, non-sporulating, mesophilic, amino acid-degrading anaerobic bacterium, designated strain OTA 102(T), was isolated from an anaerobic sequencing batch reactor treating wastewater from cooking tuna. The cells were curved rods (0.6-2.5×0.5 µm) and occurred singly or in pairs. The strain was motile by means of one lateral flagellum. Strain OTA 102(T) grew at temperatures between 30 and 45 °C (optimum 40 °C), between pH 6.0 and 8.4 (optimum pH 7.2) and NaCl concentrations between 1 and 5 % (optimum 2 %, w/v). Strain OTA 102(T) required yeast extract for growth. Serine, threonine, glycine, cysteine, citrate, fumarate, α-ketoglutarate and pyruvate were fermented. When co-cultured with Methanobacterium formicicum as the hydrogen scavenger, strain OTA 102(T) oxidized alanine, valine, leucine, isoleucine, aspartate, tyrosine, methionine, histidine and asparagine. The genomic DNA G+C content of strain OTA 102(T) was 41.7 mol%. The main fatty acid was iso-C15 : 0. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain OTA 102(T) was related to Aminobacterium colombiense and Aminobacterium mobile (95.5 and 95.2 % similarity, respectively), of the phylum Synergistetes. On the basis of phylogenetic, genetic and physiological characteristics, strain OTA 102(T) is proposed to represent a novel species of the genus Aminobacterium, Aminobacterium thunnarium sp. nov. The type strain is OTA 102(T) ( = DSM 27500(T) = JCM 19320(T)).
%G English
%L hal-01177834
%U https://hal.science/hal-01177834
%~ SDE
%~ INSU
%~ UNIV-TLN
%~ CNRS
%~ UNIV-AMU
%~ MIO
%~ OSU-INSTITUT-PYTHEAS
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