%0 Journal Article
%T Molar-Mass Analysis of Dendrigraft Poly(L-lysine) (DGL) Polyelectrolytes by SEC-MALLS: The "Cornerstone" Refractive Index Increment
%+ Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM)
%+ Ingénierie des Matériaux Polymères (IMP)
%+ COLCOM
%A Maret, Barbara
%A Crepet, Agnès
%A Faye, Clément
%A Garrelly, Laurent
%A Ladaviere, Catherine
%< avec comité de lecture
%@ 1022-1352
%J Macromolecular Chemistry and Physics
%I Wiley-VCH Verlag
%V 216
%P 95-105
%8 2015-01
%D 2015
%R 10.1002/macp.201400400
%K dendrigrafts
%K molecular-weight distribution
%K polyelectrolytes
%K refractive-index increment
%K SEC-MALLS
%Z Chemical Sciences/Polymers
%Z Chemical Sciences/Material chemistryJournal articles
%X Dendrigraft poly(L-lysine) (DGL) polyelectrolytes, obtained by iterative polycondensation of N-trifluoroacetyl-L-lysine-N-carboxyanhydride, constitute very promising candidates in many biomedical applications. In order to get a better understanding of their structure-property relationships in these applications, their absolute average molecular weights have to be accurately measured. Size-exclusion chromatography coupled to a multi-angle laser-light-scattering detector (SEC-MALLS) is known to be the most appropriate analytical tool. These measurements require the determination of the refractive index increment, dn/dc, of these highly branched polycationic macromolecules in aqueous solution. This optical property has to be measured in the same aqueous conditions as SEC-MALLS eluents. Consequently, data are determined and discussed as a function of different aqueous SEC-MALLS eluents, as well as different counter-ions of the many ammonium groups of DGL (generation 3, DGL-3, used as a model herein). The resulting number-average molecular weights, (M) over bar (n), are found to be very dissimilar when the measured dn/dc values are directly considered. In contrast, very close (M) over bar (n) values are obtained (average (M) over bar (n) = 18 700, standard error of 1110 g mol(-1)) with a low coefficient of variation for such data (ca.6% for six analyses), when the dn/dc are corrected by the exact lysine amount (measured by the total Kjeldahl nitrogen method).
%G English
%L hal-01149434
%U https://hal.science/hal-01149434
%~ UNIV-ST-ETIENNE
%~ CNRS
%~ UNIV-LYON1
%~ INSA-LYON
%~ ENSC-MONTPELLIER
%~ IBMM
%~ IMP
%~ INC-CNRS
%~ CHIMIE
%~ UNIV-MONTPELLIER
%~ INSA-GROUPE
%~ UDL
%~ UNIV-LYON
%~ UM-2015-2021
%~ TEST2-HALCNRS