Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope.

Charles-Edouard Leroux (1) , Alexei Grichine (2) , Irène Wang (3) , Antoine Delon (3)

1 MOTIV
2 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
3 MOTIV-LIPhy - Matériaux, Optique et Techniques Instrumentales pour le Vivant

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

Domaines: Biophysique [physics.bio-ph]; Biologie cellulaire

Charles-Edouard Leroux, Alexei Grichine, Irène Wang, Antoine Delon. Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope.. Optics Letters, 2013, 38 (14), pp.2401-3. ⟨hal-00958198⟩