Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS.

F. Donot (1, 2) , G. Cazals (3) , Z. Gunata (2) , D. Egron (3) , J. Malinge (1) , C. Strub (2) , A. Fontana (2) , S. Schorr-Galindo (2)

1 PERL - Total SA Pôle d'études et de recherche de Lacq
2 UMR Qualisud - Démarche intégrée pour l'obtention d'aliments de qualité
3 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45min through HPLC-APCI-MS/MS technique.

Mots clés: Neutral lipids; Microalgae; HPLC; ELSD; APCI-MS/MS; SPE

Domaines: Chimie organique

F. Donot, G. Cazals, Z. Gunata, D. Egron, J. Malinge, et al.. Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS.. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2013, 942-943, pp.98-106. ⟨10.1016/j.jchromb.2013.10.016⟩. ⟨hal-00913328⟩