Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg.

Manuel Mayr (1) , Thierry Rabilloud (2)

1 King's British Heart Foundation Centre
2 LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux

While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC [Atanassov and Urlaub, Proteomics 2013, 13, 2947-2955]. This three-step separation procedure prior to MS/MS substantially increases proteome coverage and represents a further step toward a more comprehensive analysis of complex proteomes.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Manuel Mayr, Thierry Rabilloud. Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg.. Proteomics, 2013, 13 (20), pp.2942-3. ⟨10.1002/pmic.201300400⟩. ⟨hal-00904696⟩