Heat shock proteins and cell death in situ localisation in hypopharyngeal glands of honeybee (Apis mellifera carnica) workers after imidacloprid or coumaphos treatment

Localisation in situ des protéines du choc thermique et de la mort cellulaire dans les glandes hypopharyngiennes des ouvrières de l'abeille (Apis mellifera carnica) après traitement à l'immidaclopride et au coumaphos

Maja Ivana Smodiš Škerl , Aleš Gregorc

Worker honeybees (Apis mellifera carnica Polm.) were treated with imidacloprid or coumaphos. Significant effects of treatment and treatment duration were found on hypopharyngeal glands (HPG) acinus diameter (P < 0.05). Differences in the size of acini were evident in all long term (48 h and 72 h) treatments. Short term (24 h) imidacloprid treatment induced heat shock protein 70 (Hsp 70) localisation in nuclei and cytoplasm and Hsp 90 activity was found in most cell cytoplasm. Coumaphos triggered an increased level of programmed cell death, and imidacloprid induced extended necrosis in comparison to coumaphos. In 7-12 day old workers, the level of cell death after 48 hours of imidacloprid treatment was approximately 50% and increased to all cells after 72 hours. Programmed cell death remained at the normal level of approximately 10%. Our results suggest that both pesticide treatments have an influence on the reduced size of HPG and also on the extended expression of cell death.

Mots clés: Apis mellifera; immunohistochemistry; pesticide; heat-shock; cell death<br>---<br>Apis mellifera carnica; immunohistochimie; pesticides; mort cellulaire; choc thermique

Domaines: Zoologie des invertébrés; Biodiversité; Ecologie, Environnement; Science des productions animales

Maja Ivana Smodiš Škerl, Aleš Gregorc. Heat shock proteins and cell death in situ localisation in hypopharyngeal glands of honeybee (Apis mellifera carnica) workers after imidacloprid or coumaphos treatment. Apidologie, 2010, 41 (1), ⟨10.1051/apido/2009051⟩. ⟨hal-00892015⟩