External validation of recombinant antigens for serodiagnosis of machine operator's lung. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue American Journal of Industrial Medicine Année : 2013

External validation of recombinant antigens for serodiagnosis of machine operator's lung.

Résumé

BACKGROUND: Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from Mycobacterium immunogenum isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany. METHODS: Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of M. immunogenum and three other main species isolated from contaminated metalworking fluids. RESULTS: The two recombinant antigens acyl-CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL. CONCLUSIONS: Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany. Am. J. Ind. Med. © 2013 Wiley Periodicals, Inc.

Domaines

Mycologie

Dates et versions

hal-00879496 , version 1 (04-11-2013)

Identifiants

Citer

Coralie Barrera, Gabriel Reboux, Isabel Warfolomeow, Bénédicte Rognon, Laurence Millon, et al.. External validation of recombinant antigens for serodiagnosis of machine operator's lung.. American Journal of Industrial Medicine, 2013, epub ahead of print. ⟨10.1002/ajim.22270⟩. ⟨hal-00879496⟩
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