Necrosis of HepG2 cancer cells induced by the vibration of magnetic particles

Biran Wang (1, 2) , Céline Bienvenu (2) , Juan Mendez-Garza (1) , Pascal Lançon (1) , Alexandra Madeira (1) , Pierre Vierling (2) , Christophe Di Giorgio (2) , Georges Bossis (1)

1 LPMC - Laboratoire de physique de la matière condensée
2 LCMBA - Laboratoire de Chimie des Molécules Bioactives et des Arômes

Experiments of magnetolysis, i.e., destruction of cells induced with magnetic particles (MPs) submitted to the application of a magnetic field, were conducted on HepG2 cancer cells. We herein demonstrate the usefulness of combining anisotropic MPs with an alternative magnetic field in magnetolysis. Thus, the application of an alternative magnetic field of low frequency (a few Hertz) in the presence of anisotropic, submicronic particles allowed the destruction of cancer cells "in vitro". We also show that a constant magnetic field is far less efficient than an oscillating one. Moreover, we demonstrate that, at equal particle volume, it is much more efficient to utilize spindle shaped particles rather than spherical ones. In order to get deeper insight into the mechanism of magnetolysis experiments, we performed a study by AFM, which strongly supports that the magnetic field induces the formation of clusters of particles becoming then large enough todamage cell membranes.

Mots clés: Cancer cells; Magnetic nanoparticles; Magnetolysis

Domaines: Matériaux; Biophysique [physics.bio-ph]

Biran Wang, Céline Bienvenu, Juan Mendez-Garza, Pascal Lançon, Alexandra Madeira, et al.. Necrosis of HepG2 cancer cells induced by the vibration of magnetic particles. Journal of Magnetism and Magnetic Materials, 2013, 344, pp.193-201. ⟨10.1016/j.jmmm.2013.05.043⟩. ⟨hal-00878760⟩