Tomographic diffractive microscopy of transparent samples

Bertrand Simon (1) , Matthieu Debailleul (1) , Vincent Georges (1) , Vincent Lauer (2) , Olivier Haeberlé (1)

1 MIPS - Modélisation, Intelligence, Processus et Système
2 Chercheur indépendant

We report a tomographic diffractive microscope, which permits imaging non-labelled transparent or semi-transparent samples. Based on a combination of microholography with a tomographic illumination, our set-up creates 3-D images of the index of refraction distribution within the sample. One acquires successively interferograms, rotating the illumination (the specimen being static) and using phase-shifting holography. Within the first Born approximation, each interferogram is interpreted as a subset of the Fourier transform of the specimen index of refraction distribution. The reconstruction is therefore similar to synthetic aperture imaging: one recombines the information in the Fourier space, and a final Fourier transform gives a 3-D image of the specimen. First recalling the theoretical foundations, we then describe our experiment, and show initial results obtained on biological samples.

Mots clés: Image reconstruction; tomography; Fourier optics; Holographic interferometry; other holographic techniques

Domaines: Optique [physics.optics]; Optique / photonique

Bertrand Simon, Matthieu Debailleul, Vincent Georges, Vincent Lauer, Olivier Haeberlé. Tomographic diffractive microscopy of transparent samples. European Physical Journal: Applied Physics, 2008, 44, pp.29-35. ⟨10.1051/epjap:2008049⟩. ⟨hal-00869566⟩