Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation

Stanislas Vertu (1) , Jens Flügge (1) , Jean-Jacques Delaunay (2) , Olivier Haeberlé (3)

1 PTB - Physikalisch-Technische Bundesanstalt [Braunschweig]
2 Department of Mechanical Engineering
3 MIPS - Modélisation, Intelligence, Processus et Système

Tomographic Diffractive Microscopy is a technique, which permits to image transparent living specimens in three dimensions without staining. It is commonly implemented in two configurations, by either rotating the sample illumination keeping the specimen fixed, or by rotating the sample using a fixed illumination. Under the first-order Born approximation, the volume of the frequency domain that can be mapped with the rotating illumination method has the shape of a "doughnut", which exhibits a so-called "missing cone" of non-captured frequencies, responsible for the strong resolution anisotropy characteristic of transmission microscopes. When rotating the sample, the resolution is almost isotropic, but the set of captured frequencies still exhibits a missing part, the shape of which resembles that of an apple core. Furthermore, its maximal extension is reduced compared to tomography with rotating illumination. We propose various configurations for tomographic diffractive microscopy, which combine both approaches, and aim at obtaining a high and isotropic resolution. We illustrate with simulations the expected imaging performances of these configurations.

Mots clés: tomography; holography; Fourier optics; image reconstruction

Domaines: Optique / photonique

Stanislas Vertu, Jens Flügge, Jean-Jacques Delaunay, Olivier Haeberlé. Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation. Central European Journal of Physics, 2011, 9 (4), pp.969-974. ⟨10.2478/s11534-011-0018-3⟩. ⟨hal-00851880⟩