%0 Journal Article
%T Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel
%+ Laboratoire de Bioingénierie et NanoSciences (LBN)
%+ Laboratoire Charles Coulomb (L2C)
%A Salehi, Hamideh
%A Middendorp, Elodie
%A Panayotov, Ivan
%A Collard Dutilleul, Pierre-Yves
%A Vegh, Attila Gergely
%A Ramakrishnan, Satish
%A Gergely, Csilla
%A Cuisinier, Frédéric J. G.
%< avec comité de lecture
%@ 1083-3668
%J Journal of Biomedical Optics
%I Society of Photo-optical Instrumentation Engineers
%V 18
%N 5
%P 056010
%8 2013-05-09
%D 2013
%R 10.1117/1.JBO.18.5.056010
%K Apoptosis
%K Raman microscopy
%K paclitaxel
%K K-mean cluster
%K correlation coefficient
%K living cell.
%K living cell
%Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Journal articles
%X Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cyto- plasm, indicating cell apoptosis via a caspase independent pathway.
%G English
%L hal-00822202
%U https://hal.science/hal-00822202
%~ CNRS
%~ UNIV-MONTP1
%~ L2C
%~ MIPS
%~ BS
%~ UNIV-MONTPELLIER
%~ LBN
%~ UM1-UM2
%~ UM-2015-2021