%0 Journal Article %T Phytoplankton group dynamics in the Bay of Marseilles during a 2-year survey based on analytical flow cytometry %+ Laboratoire d'océanographie et de biogéochimie (LOB) %A Grégori, Gérald %A Colosimo, A %A Denis, Michel %< avec comité de lecture %Z MIO:01-021 %@ 0196-4763 %J Cytometry %I Wiley %V 44 %N 3 %P 247-256 %8 2001-07-01 %D 2001 %R 10.1002/1097-0320(20010701)44:3<247::AID-CYTO1117>3.0.CO;2-Z %K flow cytometry %K microorganism monitoring %K coastal waters %K artificial neural network analysis %Z Sciences of the Universe [physics]/Ocean, AtmosphereJournal articles %X Background: The Bay of Marseilles is under the influence of a large urban concentration and its maritime activities. All of them discharge compounds (hydrocarbons, excess nutrients, heavy metals, chemicals, etc.) that can alter the marine ecosystem. To investigate whether ultraphyto- plankton ( , 10 m m) could be used as biosensors for their own ecosystem, a 2-year survey was conducted in the Bay of Marseilles. Methods: Seven stations monitored water mass and po- tential anthropic effects in the bay. Seawater samples were collected monthly or bimonthly at three depths, prefiltered, fixed, and kept in liquid nitrogen until flow cytometric analysis. Results: Five categories were created: Prochlorococcus, picoeukaryotes ( , 2 m m), nanoeukaryotes I (2- 6 m m), nanoeukaryotes II (6 -10 m m), and Synechococcus ( , 1.5 m m). Artificial neural network analysis (Kohonen self-or- ganizing maps) produced the same number of clusters as cluster analysis with Winlist software (Verity Software House). Conclusions: In addition to the wide variabilities in abun- dance and biomass, there were a strong seasonal signal and sporadic events. Lessons are derived from this study for future monitoring of marine microorganisms. Cytometry 44:247-256, 2001. © 2001 Wiley-Liss, Inc. %G English %L hal-00802074 %U https://hal.science/hal-00802074 %~ INSU %~ CNRS %~ UNIV-AMU %~ GIP-BE %~ LOPB %~ ACL_CFOM