Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability

Benjamin Erable (1) , Isabelle Goubet (1) , Sylvain Lamare (1) , Marie-Dominique Legoy (1) , Thierry Maugard (1)

1 LBCBO - Laboratoire de Biotechnologies et de Chimie Bio-organique

Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9.0. The most effective dehalogenase activity for 1-chlorobutane conversion was detected with Rhodococcus erythropolis NCIMB13064 and Escherichia coli BL21 (DE3) (DhaA). The presence of 2-chlorobutane or propanal in the aqueous media could inhibit the 1-chlorobutane transformation.

Mots clés: Haloalkane dehalogenase; Rhodococcus erythropolis NCIMB13064; Sphingomonas paucimobilis UT26; Xanthobacter autotrophicus GJ10

Domaines: Génie chimique; Biodiversité et Ecologie

Benjamin Erable, Isabelle Goubet, Sylvain Lamare, Marie-Dominique Legoy, Thierry Maugard. Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability. Chemosphere, 2006, vol. 65, pp. 1146-1152. ⟨10.1016/j.chemosphere.2006.04.007⟩. ⟨hal-00782648⟩