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Article Dans Une Revue Biophysical Chemistry Année : 2011

Structural characterization of liposomes made of diether archaeal lipids and dipalmitoyl-L-α-phosphatidylcholine

Résumé

The physicochemical properties of binary lipid mixtures of diether C lipids and dipalmitoyl-L-α-phosphatidylcholine (DPPC) were studied using photon correlation, fluorescence spectroscopy, electron paramagnetic resonance spectroscopy, and transmission electron microscopy. These two types of lipids can be mixed at all molar ratios to form unilamellar and multilamellar liposomes. Fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatrien in mixed liposomes indicates that the abrupt changes in order parameter in the hydrophobic part of bilayer membranes made of DPPC lipids disappears with increasing mol%C lipids. Electron paramagnetic resonance spectroscopy shows that at temperatures below 50°C, the interfacial regions of membrane bilayer of mixed liposomes is more fluid than for pure DPPC liposomes, while at higher temperatures, the impact of the long isoprenoid chains on the membrane stability becomes more pronounced. Photon correlation spectroscopy and transmission electron microscopy show that mixed liposomes do not fuse or aggregate, even after 41days at 4°C.
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Dates et versions

hal-00778543 , version 1 (21-01-2013)

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Dejan Gmajner, Pegi Ahlin Grabnar, Magda Tušek Žnidarič, Jasna Štrus, Marjeta Šentjurc, et al.. Structural characterization of liposomes made of diether archaeal lipids and dipalmitoyl-L-α-phosphatidylcholine. Biophysical Chemistry, 2011, 158 (2-3), pp.150. ⟨10.1016/j.bpc.2011.06.014⟩. ⟨hal-00778543⟩

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