%0 Journal Article
%T A rapid and reversible colorimetric assay for the characterization of aminated solid surfaces
%+ Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM)
%+ sSE 2011
%A Coussot, Gaëlle
%A Perrin, Catherine
%A Moreau, Thomas
%A Dobrijevic, M.
%A Le Postollec, A.
%A Vandenabeele-Trambouze, Odile
%< avec comité de lecture
%@ 1618-2642
%J Analytical and Bioanalytical Chemistry
%I Springer Verlag
%V 399
%N 3
%P 1061-1069
%8 2011
%D 2011
%R 10.1007/s00216-010-4363-7
%K Coomassie blue
%K ADECA
%K quantification
%K amine density
%K solid support characterization
%Z Chemical Sciences/Organic chemistryJournal articles
%X The covalent immobilization of synthetic or natural macromolecular compounds containing amino groups onto polystyrene (PS) solid surfaces is of great interest in diagnostic applications. A sensitive assay allowing the determination of reactive end groups is therefore a powerful tool for predicting the performance of the active surface. Recently, we reported the use of the Coomassie Brilliant Blue (CBB) colorimetric reagent to quantify protonated groups (N+) in linear and dendritic structures in solution (Coussot G et al. Polym Int 58(5):511-518). In this work, a simple method using CBB dye for the characterization of PS aminated solid-surfaces is developed. The proposed Amino Density Estimation by Colorimetric Assay (ADECA) method is based on the reversible complexation of the dye with the N+ groups on solid surfaces. The assay measures the released dye thanks to the use of a unique sodium carbonate-methanol buffer. Thereby, for the first time, the same surface can be used for characterization and for further coupling applications. A surface density of 4 N + groups per nm2 can be measured in PS microwell format, the whole characterization being done within 30 min. Performances of this new colorimetric-based method are detailed. The ADECA method is further demonstrated to be useful for the characterization of aminated polypropylene, and glass materials with various sizes and shapes.
%G English
%L hal-00553885
%U https://hal.science/hal-00553885
%~ INSU
%~ CNRS
%~ ENSC-MONTPELLIER
%~ L3AB
%~ IBMM
%~ INC-CNRS
%~ CHIMIE
%~ UNIV-MONTPELLIER
%~ UM-2015-2021
%~ TEST2-HALCNRS