Investigation of Silicon-Based Nanostructure Morphology and Chemical Termination on Laser Desorption Ionization Mass Spectrometry Performance

Mathieu Dupré (1) , Christine Enjalbal (1) , Sonia Cantel (1) , Jean Martinez (1) , Nacéra Megouda (2, 3) , Toufik Hadjersi (3) , Rabah Boukherroub (2) , Yannick Coffinier (2)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 IRI - Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq]
3 Unité de Développement de la Technologie du Silicium

We have evaluated the laser desorption ionization mass spectrometry (LDI-MS) performance of six nanostructured silicon surfaces of different morphologies and chemical functionalizations. The substrates have been synthesized either by metal-assisted etching method or by vapor−liquid−solid (VLS) growth technique. In addition to the commercial nanostructured silicon-based surface (NALDI) target plates, serving as reference, the homemade surfaces have been evaluated in mass spectrometry experiments conducted with peptide solutions mimicking tryptic digests. LDI surfaces synthesized by metal-assisted etching method were the most efficient in terms of signal intensities and number of detected peptides. The surface providing the best LDI-MS performance was composed of two nanostructured layers. Interestingly, we also observed a significant influence of the type of organic coating (hydrocarbon vs fluorocarbon) on peptide ionization discrimination.

Domaines: Chimie organique

Mathieu Dupré, Christine Enjalbal, Sonia Cantel, Jean Martinez, Nacéra Megouda, et al.. Investigation of Silicon-Based Nanostructure Morphology and Chemical Termination on Laser Desorption Ionization Mass Spectrometry Performance. Analytical Chemistry, 2012, 84, pp.10637-10644. ⟨10.1021/ac3021104⟩. ⟨hal-00771400⟩