%0 Journal Article
%T Thick collagen-based 3D matrices including growth factors to induce neurite outgrowth.
%+ Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM)
%+ Mécanismes moléculaires dans les démences neurodégénératives (MMDN)
%+ Laboratoire Charles Coulomb (L2C)
%A Labour, M.-N.
%A Banc, Amélie
%A Tourrette, Audrey
%A Cunin, Frédérique
%A Verdier, Jean-Michel
%A Devoisselle, Jean-Marie
%A Marcilhac, Anne
%A Belamie, Emmanuel
%< avec comité de lecture
%@ 1742-7061
%J Acta Biomaterialia
%I Elsevier
%V 8
%N 9
%P 3302-12
%8 2012-09
%D 2012
%Z 1211.0301
%R 10.1016/j.actbio.2012.05.015
%M 22617741
%Z Chemical Sciences/Material chemistry
%Z Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]Journal articles
%X Designing synthetic microenvironments for cellular investigations is a very active area of research at the crossroads of cell biology and materials science. The present work describes the design and functionalization of a three-dimensional (3D) culture support dedicated to the study of neurite outgrowth from neural cells. It is based on a dense self-assembled collagen matrix stabilized by 100-nm-wide interconnected native fibrils without chemical crosslinking. The matrices were made suitable for cell manipulation and direct observation in confocal microscopy by anchoring them to traditional glass supports with a calibrated thickness of ∼50μm. The matrix composition can be readily adapted to specific neural cell types, notably by incorporating appropriate neurotrophic growth factors. Both PC-12 and SH-SY5Y lines respond to growth factors (nerve growth factor and brain-derived neurotrophic factor, respectively) impregnated and slowly released from the support. Significant neurite outgrowth is reported for a large proportion of cells, up to 66% for PC12 and 49% for SH-SY5Y. It is also shown that both growth factors can be chemically conjugated (EDC/NHS) throughout the matrix and yield similar proportions of cells with longer neurites (61% and 52%, respectively). Finally, neurite outgrowth was observed over several tens of microns within the 3D matrix, with both diffusing and immobilized growth factors.
%G English
%2 https://hal.science/hal-00747635/document
%2 https://hal.science/hal-00747635/file/Labour_et_al_Acta_Biomat_2012.pdf
%L hal-00747635
%U https://hal.science/hal-00747635
%~ INSERM
%~ EPHE
%~ CNRS
%~ UNIV-MONTP1
%~ UNIV-MONTP2
%~ ENSC-MONTPELLIER
%~ ICG
%~ MMDN
%~ L2C
%~ INC-CNRS
%~ PSL
%~ MIPS
%~ BS
%~ CHIMIE
%~ UNIV-MONTPELLIER
%~ TEST-DEV
%~ EPHE-PSL
%~ UM1-UM2
%~ UM-2015-2021