Development of a new screening method for the detection of antibiotic residues in muscle tissues using liquid chromatography and high resolution mass spectrometry with a LC-LTQ-Orbitrap instrument.
Résumé
In the present work, a liquid chromatography- high resolution mass spectrometry method was developed for the screening in meat of a wide range of antibiotics used in veterinary medicine. Full scan mode under high resolution mass spectral conditions using LTQ-Orbitrap mass spectrometer with resolving power 60.000 FWHM was applied for analysis of the samples. Samples were prepared using two extractions protocols prior to LC-MS analysis. The scope of the method focuses on the screening of the following main families of antibacterial veterinary drugs: penicillins, cephalosporins, sulphonamides, macrolides, tetracyclines, aminoglucosides and quinolones. Compounds were successfully identified in spiked samples by their accurate mass and LC retention times from the acquired full-scan chromatogram. An automatic process of the data using ToxId software allows a rapid treatment of the data. Analyses of muscle tissues from real samples collected on antibiotic treated animals have been carried out using the above methodology and antibiotic residues have been identified unambiguously. Further analysis of the data of the real samples allowed the identification of the targeted antibiotic residues but also the non targeted compounds, such as some of their metabolites.
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