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Article Dans Une Revue European Physical Journal: Applied Physics Année : 2011

Antigen-antibody selective recognition using LiTaO SH-SAW sensors: investigations on macromolecules effects on binding kinetic constants

Y. Bergaoui
  • Fonction : Auteur
C. Zerrouki
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N. Fourati
  • Fonction : Auteur
J. M. Fougnion
  • Fonction : Auteur
A. Abdelghani
  • Fonction : Auteur

Résumé

A gravimetric surface acoustic wave (SAW) biosensor, based on the biotin-streptavidin and antistreptavidin-streptavidin recognitions, has been carried out. A network analyser and a pulse excitation technique were used to monitor both amplitude and phase changes. The SAW biosensor presented a total selective recognition of streptavidin-antistreptavidin and HRPstreptavidin-antistreptavidin. The presence of HRP (Horseradish peroxidase) affects neither the selectivity nor the sensitivity (of order of 0.25°/nM) of the biosensor, nevertheless, it causes a reduction of binding kinetics by a factor ranging between 2 to 5, as well as a diminution of antistreptavidin saturation concentration (of 40%). Results showed that equilibrium constants can be different, depending on evaluation method (from saturation values or from linear part of the output signal variation according to solution concentration).

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Dates et versions

hal-00736288 , version 1 (28-09-2012)

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Y. Bergaoui, C. Zerrouki, N. Fourati, J. M. Fougnion, A. Abdelghani. Antigen-antibody selective recognition using LiTaO SH-SAW sensors: investigations on macromolecules effects on binding kinetic constants. European Physical Journal: Applied Physics, 2011, 56 (1), pp.13705. ⟨10.1051/epjap/2011100225⟩. ⟨hal-00736288⟩

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