A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: Application to a field survey.

Agathe Nkouawa (1) , Yasuhito Sako (1) , Tiaoying Li (2) , Xingwang Chen (2) , Minoru Nakao (1) , Tetsuya Yanagida (1) , Munehiro Okamoto (3) , Patrick Giraudoux (4) , Francis Raoul (4) , Kazuhiro Nakaya (5) , Ning Xiao (2) , Jiamin Qiu (2) , Dongchuan Qiu (2) , Philip S Craig (6) , Akira Ito (1)

1 Asahikawa Medical University
2 Sichuan Provincial Center for Diseases Control and Prevention
3 Primate Research Institute
4 LCE - Laboratoire Chrono-environnement (UMR 6249)
5 Department of Parasitology
6 University of Salford

In this study, we applied a loop-mediated isothermal amplification method for identification of human Taenia tapeworms in Tibetan communities in Sichuan, China. Out of 51 proglottids recovered from 35 carriers, 9, 1, and 41 samples were identified as Taenia solium, Taenia asiatica and Taenia saginata, respectively. Same results were obtained afterwards in the laboratory, except one sample. These results demonstrated that the LAMP method enabled rapid identification of parasites in the field surveys, which suggested that this method would contribute to the control of Taenia infections in endemic areas.

Domaines: Parasitologie

Agathe Nkouawa, Yasuhito Sako, Tiaoying Li, Xingwang Chen, Minoru Nakao, et al.. A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: Application to a field survey.. Parasitology International, 2012, 61 (4), pp.723-725. ⟨10.1016/j.parint.2012.06.001⟩. ⟨hal-00727102⟩