Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria

Mariam Fadli (1) , Jacqueline Chevalier , Asmaa Saad (1) , Nour-Eddine Mezrioui (1) , Lahcen Hassani (1) , Jean-Marie Pages

1 Faculty of Science

Bacterial drug resistance is a worrying public health problem. Antibiotic efflux is a major non-specific resistance mechanism used by bacteria, and efflux pumps are involved in the low-level susceptibility of various important Gram-negative pathogens. Use of molecules that can block bacterial pumps is an attractive strategy, but several studies report only partial efficacy owing to limits of these molecules (stability, selectivity, bioavailability, toxicity, etc.). The objective of this study was to search for natural sources of molecules able to inhibit efflux pump systems of resistant Gram-negative bacteria (, , , serotype Typhimurium and ). The results indicate that the studied essential oils exhibit interesting activity against the tested bacteria. This activity was significantly enhanced in the presence of an efflux pump inhibitor such as phenylalanine arginyl β-naphthylamide (PAβN). The role of lipopolysaccharide (LPS) structure in the effect of essential oils was also reported in LPS deep-rough mutants. In addition, essential oils of and , used at a low concentration (a fraction of the minimum inhibitory concentration), are able to significantly increase chloramphenicol susceptibility of several resistant isolates. These results demonstrate that these essential oils can alter efflux pump activity and may be attractive candidates to develop new drugs for chemosensitising multidrug-resistant strains to clinically used antibiotics.

Mots clés: Antibiotics; Antibiotic resistance; Chemosensitisers; Efflux pumps inhibitors; Efflux systems; Essential oils; Gram-negative bacteria; Multidrug resistance

Domaines: Microbiologie et Parasitologie

Mariam Fadli, Jacqueline Chevalier, Asmaa Saad, Nour-Eddine Mezrioui, Lahcen Hassani, et al.. Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria. International Journal of Antimicrobial Agents, 2011, 38 (4), pp.325. ⟨10.1016/j.ijantimicag.2011.05.005⟩. ⟨hal-00725397⟩