Cross-reactivity of some commercially available DON and ZEN immunoaffinity columns to DON and ZEN conjugated forms and metabolites

Aleksandrs Versilovskis (1) , Bart Huybrecht (1) , Emmanuel K Tangni (1) , Pussemier Luc (1) , Sarah de Saeger (2) , Alfons Callebaut (1)

1 Unit of Toxins and Natural Compounds
2 Laboratory of Food Analysis

Abstract Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) and metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) and nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra performance liquid chromatography - tandem mass spectrometry method. The DON IACs showed cross-reactivity for nearly all DON derivatives tested. The ZEN IACs showed limited cross-reactivity to some of the ZEN derivatives. The IACs were evaluated for their potential use as sample clean-up for mycotoxins in serum.

Mots clés: Life Sciences

Aleksandrs Versilovskis, Bart Huybrecht, Emmanuel K Tangni, Pussemier Luc, Sarah de Saeger, et al.. Cross-reactivity of some commercially available DON and ZEN immunoaffinity columns to DON and ZEN conjugated forms and metabolites. Food Additives and Contaminants, 2011, pp.1. ⟨10.1080/19440049.2011.603364⟩. ⟨hal-00722242⟩