Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares

P. Casagrande Proietti (1) , A. Bietta (1) , G. Coppola , M. Felicetti , R.F. Cook (2) , M. Coletti , M.L. Marenzoni , F. Passamonti

1 Department of Biopathological Science and Hygiene of Food and Animal Production
2 Department of Veterinary Science

The objective of this manuscript was to validate published PCR-based methods for detection of β-haemolytic Streptococci by comparison with established bacteriological techniques using 85 clinical isolates recovered from uterine swabs of mares with clinical signs of endometritis and to determine the distribution of SeeL/SeeM and SzeL/SzeM superantigens in isolates of subsp. () and subsp. (). The conventional bacteriological techniques showed the vast majority of these isolates (78) were with just 5 subsp. () and 2 strains detected. The PCR analyses confirmed the bacteriological results demonstrating the reliability of the 16SrRNA PCR assay for detecting , the multiplex PCR for differentiating between , and , and PCR assays based on streptokinase genes for identification of . PCRs for genes encoding superantigens revealed and specific amplicons with size of approximately 800 and 810bp respectively for the strains and for 2 strains. To our knowledge, this is the first report of s and possession by isolates derived from endometritis in mares.

Mots clés: superantigens; β-haemolytic Streptococci; endometritis; horses; bacteriological method; PCR; superantigens.

Domaines: Médecine vétérinaire et santé animale

P. Casagrande Proietti, A. Bietta, G. Coppola, M. Felicetti, R.F. Cook, et al.. Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares. Veterinary Microbiology, 2011, 152 (1-2), pp.126. ⟨10.1016/j.vetmic.2011.04.009⟩. ⟨hal-00719072⟩