Molecular epidemiology of in dairy and beef calves

E.J. Hotchkiss (1) , J.C. Hodgson (1) , E. Schmitt-van De Leemput , M.P. Dagleish (1) , R.N. Zadoks (1)

1 MRI - Moredun Research Institute [Penicuik, UK]

The molecular epidemiology of has rarely been studied at the farm level in cattle. The aim of this study was to determine whether single or multiple strains of tend to exist within farms. Molecular characterisation was carried out on isolates obtained from nasal swabs from 105 calves from 32 randomly selected beef and dairy farms located throughout Scotland, and from 131 calves from 20 farms in the Mayenne region of France, where sampling occurred in response to respiratory disease outbreaks. isolates were characterised by random-amplified polymorphic DNA (RAPD) typing and pulsed-field gel electrophoresis (PFGE) using restriction enzyme . In addition, isolates representative of each farm/RAPD profile combination were typed by multilocus sequence typing (MLST).

Mots clés: epidemiology; cattle; respiratory; PFGE; RAPD

Domaines: Médecine vétérinaire et santé animale

E.J. Hotchkiss, J.C. Hodgson, E. Schmitt-van De Leemput, M.P. Dagleish, R.N. Zadoks. Molecular epidemiology of in dairy and beef calves. Veterinary Microbiology, 2011, 151 (3-4), pp.329. ⟨10.1016/j.vetmic.2011.03.018⟩. ⟨hal-00717086⟩