Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer

Jinyu Wang (1, 2) , J.-F. Leger (2) , Jonas Binding (1, 2, 3) , A.C. Boccara (1) , Sylvain Gigan (1) , Laurent Bourdieu (2)

1 IL - Institut Langevin - Ondes et Images (UMR7587)
2 IBENS - Institut de biologie de l'ENS Paris
3 Max Planck Institute for Medical Research [Heidelberg]

Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so far only in weakly scattering samples. We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope. In the highly scattering rat brain tissue, where multiply scattered photons falling within the temporal gate of the CGWS can no longer be neglected, we have measured known defocus and spherical aberrations up to a depth of 400μm.

Mots clés: Active or adaptive optics; Wavefront sensing; Imaging through turbid media.

Domaines: Optique [physics.optics]; Imagerie

Jinyu Wang, J.-F. Leger, Jonas Binding, A.C. Boccara, Sylvain Gigan, et al.. Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer. Biomedical optics express, 2012, 3 (10), pp.2510. ⟨10.1364/BOE.3.002510⟩. ⟨hal-00716152v2⟩