%0 Journal Article
%T Optimized conditions for the synthesis of vanillic acid under hypersaline conditions by Halomonas elongata DSM 2581(T) resting cells
%+ Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds
%A Abdelkafi, Slim
%A Labat, Marc
%A Gam, Zouhaier Ben Ali
%A Lorquin, Jean
%A Casalot, Laurie
%A Sayadi, Sami
%< avec comité de lecture
%Z MIO:08-100
%J WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
%V 24
%P 675-680
%8 2008
%D 2008
%R 10.1007/s11274-007-9523-3
%K hypersaline conditions
%K resting cells
%K vanillic acid
%K Halomonas elongata
%K PSEUDOMONAS-FLUORESCENS STRAIN
%K FERULIC ACID
%K FATTY-ACIDS
%K BIOCONVERSION
%K CONVERSION
%K TYROSOL
%K HYDROXYTYROSOL
%K BACTERIA
%K BIOTRANSFORMATION
%K METABOLISM
%Z Environmental Sciences/Biodiversity and EcologyJournal articles
%X During growth on ferulic acid, Halomonas elongata DSM 2581(T) was capable of promoting the formation of a significant amount of vanillic acid. The products were confirmed by high-performance liquid chromatography and gas chromatography mass-spectrometry analyses. To enhance the formation of vanillic acid and prevent its degradation, a resting-cell method using Halomonas elongata was developed. The growth state of the culture utilized for biomass production, the concentration of the biomass, the amount of ferulic acid that was treated and the reutilization of the biomass were optimized. The optimal yield of vanillic acid (82%) was obtained after a 10-h reaction using 10 mM ferulic acid and 5 g/l of cell pregrown on ferulic acid and harvested at the end of the exponential phase.
%G English
%L hal-00713655
%U https://hal.science/hal-00713655
%~ SDE
%~ UNIV-AMU
%~ GIP-BE
%~ LMBEC