Sphinganine and sphingosine levels and ratio in urine and blood samples from a Catalonian population (Spain).

Germán Cano-Sancho (1) , S. Marin , V. Sanchis , C. Colom , M.B. Coronel , A.J. Ramos

1 LABERCA - Laboratoire d'étude des Résidus et Contaminants dans les Aliments

Fumonisins occur mainly in maize, and produce alterations on sphingolipid metabolism, unbalancing sphinganine (Sa) / sphingosine (So) ratio. This alteration has been proposed as a biomarker of fumonisin exposure. The objective of the present work was to study the urinary and plasmatic levels of Sa, So as well as, the ratio Sa/So from a sample of Catalonian population exposed at low levels of fumonisins. Firstly, plasmatic and urinary Sa and So levels and the ratio Sa/So were compared between two population groups, and after, urinary Sa and So levels from corn-food consumers and a control group were monitored during two weeks under controlled intake of corn-foods. Sa and So levels were determined in urine and blood samples using validated methods using HPLC with fluorescence detection. Significant differences were not found in urine samples when Sa/So ratios were compared from corn-food consumers and non consumers, while significant differences were found in urine and plasma samples but evidences of mechanism of action of fumonisins were not apparent. Through time-course study, we have narrowed down the day in which the maximum alteration of Sa/So ratio should be expected in humans. In this paper we have reported some useful information to improve the design of studies to validate the ratio Sa/So as a possible biomarker of fumonisin exposure.

Mots clés: Life Sciences

Domaines: Sciences du Vivant [q-bio]

Germán Cano-Sancho, S. Marin, V. Sanchis, C. Colom, M.B. Coronel, et al.. Sphinganine and sphingosine levels and ratio in urine and blood samples from a Catalonian population (Spain).. Food Additives and Contaminants, 2011, 28 (8), pp.1. ⟨10.1080/19440049.2011.576437⟩. ⟨hal-00713059⟩