Development and Validation of a Potentiometric Biosensor Assay for Tylosin with Demonstrated Applicability for Detection of Two Other Antimicrobial Growth Promoter Compounds in Feedstuffs
Résumé
A potentiometric biosensor assay based on a commercially available polyclonal antibody has been developed to detect tylosin residues in animal feed. The method can be used as a rapid (less than 45 minutes) laboratory-based procedure or, as a portable field-test for the simultaneous measurement of up to 12 different samples. For both procedures the qualitative detection capability (CC) for tylosin was determined as 0.2 mg kg-1 in a range of animal feeds with a measurement repeatability at concentrations between 0.2 and 4 mg kg-1 of ≤13% %Coefficient of Variation (%CV). The field-test format was capable of detecting tylosin residues at operating (external air) temperatures ranging between +4 to 37oC although some reduction in signal was observed at the lower temperatures. The laboratory-based tylosin assay was evaluated using 16 medicated and 22 non-medicated feeds and found to give comparable data to a confirmatory method based upon Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS). The potential to develop a multi-probe format assay for the simultaneous detection of tylosin, spiramycin and virginiamycin was also demonstrated. Cross-validation in a second laboratory showed the assay to be transferable, reliable and robust.
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