Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray

Mark Melville (1) , Padraig Doolan (2) , William Mounts (3) , Niall Barron (2) , Louane Hann (1) , Mark Leonard (1) , Martin Clynes (2) , Tim Charlebois (1)

1 Pfizer
2 National Institute for Cellular Biotechnology
3 Pfizer

The Chinese hamster ovary (CHO) cell line is one of the most widely used mammalian cell lines for biopharmaceutical production. We have developed and characterized a gene expression microarray (WyeHamster2a) specific for CHO cells that has enabled the study of ~3,500 sequences. Analysis of multiple sets of replicate scans showed that data derived from the WyeHamster2a array is highly reproducible confirming it as a robust tool for profiling. Twelve gene sequences were selected for follow-up RT-qPCR to confirm the accuracy and precision of the microarray results. In all but the most subtle gene expression differences, the microarray proved to be a reliable measure of differential gene expression. Finally, we were able to quantify the difference between using a bona fide CHO-specific microarray for profiling CHO cells versus an alternate, commercially available, rodent microarray such as a mouse or rat-specific format.

Mots clés: Affymetrix; Chinese hamster ovary; Microarray

Domaines: Biotechnologie; Biotechnologies

Mark Melville, Padraig Doolan, William Mounts, Niall Barron, Louane Hann, et al.. Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray. Biotechnology Letters, 2011, 33 (9), pp.1773-1779. ⟨10.1007/s10529-011-0628-2⟩. ⟨hal-00694736⟩