%0 Book Section
%T Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single‐Cell Level by Flow Cytometry
%+ Laboratoire de MicrobiologiE de Géochimie et d'Ecologie Marines (LMGEM)
%+ Institut méditerranéen d'océanologie (MIO)
%+ Biological Centre of the Academy of Sciences
%A Duhamel, Solange
%A Grégori, Gérald
%A van Wambeke, France
%A Nedoma, Jiri
%Z MIO:12-013
%B Current Protocols in Cytometry
%E J. Paul Robinson
%E Zbigniew Darzynkiewicz
%E et al.
%I John Wiley & Sons, Inc.
%P 49:11.18.1-11.18.8.
%8 2009-01-01
%D 2009
%K aquatic heterotrophic bacteria
%K phosphatase activity
%K ectoenzyme
%K flow cytometry
%K cell concentration
%K functional measurement
%Z Environmental Sciences/Biodiversity and Ecology
%Z Life Sciences [q-bio]/Ecology, environment/Ecosystems
%Z Life Sciences [q-bio]/Biotechnology
%Z Computer Science [cs]/Biotechnology
%Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyBook sections
%X Monitoring cell activity using substrates, which turn fluorescent due to biological activity, allows observing the presence and dynamics of sub-populations, and provides a very valuable insight in ecological studies. The phosphatase substrate ELF97 phosphate (ELF-P) is a useful tool to detect and quantify phosphatase activity (PA) of microorganisms at the single-cell level. Most of the studies dealing with PA at the single-cell level focus on autotrophic cells and only few concern heterotrophic prokaryotes (referred as bacteria in the text). While flow cytometry is a promising tool to assess the single-cell analysis, only microscopy tools have been used until now to measure the ELF labeling associated with bacteria expressing PA. Therefore, we have developed a new protocol that enables the detection of ELF alcohol (ELFA), the product of ELF-P hydrolysis, making possible the specific identification of bacteria showing PA using flow cytometry. Keywords:
%G English
%L hal-00692148
%U https://hal.science/hal-00692148
%~ SDE
%~ INSU
%~ UNIV-TLN
%~ CNRS
%~ UNIV-AMU
%~ MIO
%~ OSU-INSTITUT-PYTHEAS
%~ GIP-BE
%~ LMGEM
%~ MIO-CYBELE