Antibody responses in furunculosis patients vaccinated with autologous formalin-killed

S. Holtfreter (1, 2) , J. Jursa-Kulesza (3) , H. Masiuk (3) , N. J. Verkaik (4) , C. Vogel (4) , J. Kolata (1) , M. Nowosiad (3) , L. Steil (5) , W. Wamel (4) , A. Belkum (4) , U. Völker (5) , S. Giedrys-Kalemba (3) , B. M. Bröker (1)

1 Institute of Immunology and Transfusion Medicine
2 Department of Molecular Medicine and Pathology
3 Department of Microbiology and Immunology
4 Department of Medical Microbiology and Infectious Diseases
5 Interfaculty Institute of Genetics and Functional Genomics

Autologous vaccines (short: autovaccines) have been used since the beginning of the 20th century to treat chronic staphylococcal infections, but their mechanisms of action are still obscure. This prospective pilot study involved four patients with furunculosis who were vaccinated with autologous formalin-killed cells. Vaccines were individually prepared from the infecting strain and repeatedly injected subcutaneously in increasing doses over several months. We characterized the virulence gene repertoire and genotype of the infecting and colonising strains. Serum antibody responses to secreted and surface-bound bacterial antigens were determined by two-dimensional immunoblotting and flow-cytometry based assays (Luminex®). All patients reported clinical improvement. Molecular characterization showed that all strains isolated from one patient over time belonged to the same clone. Already before treatment, there was robust antibody binding to a broad range of staphylococcal antigens. Autovaccination moderately boosted the IgG response to extracellular antigens in two patients, while the antibody response of the other two patients was not affected. Similarly, vaccination moderately enhanced the antibody response against some staphylococcal surface proteins, e.g. ClfA, ClfB, SdrD and SdrE. In summary, autovaccination only slightly boosted the pre-existing serum antibody response, predominantly to bacterial surface antigens.

Mots clés: Life Sciences

Domaines: Maladies infectieuses

S. Holtfreter, J. Jursa-Kulesza, H. Masiuk, N. J. Verkaik, C. Vogel, et al.. Antibody responses in furunculosis patients vaccinated with autologous formalin-killed. European Journal of Clinical Microbiology and Infectious Diseases, 2011, 30 (6), pp.707-717. ⟨10.1007/s10096-010-1136-3⟩. ⟨hal-00690187⟩