Anthracene Modified Oligonucleotides as Fluorescent DNA Mismatch Sensors: Discrimination Between Various Base Pair Mismatches

Jim Tucker (1) , Eva Hyde (2) , Christian Ludwig (3) , John Zhao (1)

1 School of Chemistry [Birmingham]
2 Biosciences
3 University of Birmingham [Birmingham]

A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch and decreasing for CT and CC mismatches.

Mots clés: Physical Sciences

Domaines: Autre

Jim Tucker, Eva Hyde, Christian Ludwig, John Zhao. Anthracene Modified Oligonucleotides as Fluorescent DNA Mismatch Sensors: Discrimination Between Various Base Pair Mismatches. Supramolecular Chemistry, 2011, 23 (03-04), pp.273-277. ⟨10.1080/10610278.2010.523117⟩. ⟨hal-00687341⟩