A cathelicidin-2-derived peptide effectively impairs biofilms

E. Margo Molhoek (1) , Albert van Dijk (1) , Edwin J.A. Veldhuizen (1) , Henk P. Haagsman (1) , Floris J. Bikker (2)

1 Faculty of Veterinary Medicine
2 Department of Oral Biochemistry

is a major cause of nosocomial infections owing to its ability to form biofilms on the surface of medical devices. Biofilms are surface-adhered bacterial communities. In mature biofilms these communities are encased in an extracellular matrix composed of bacterial polysaccharides, proteins and DNA. The antibiotic resistance of bacteria present in biofilms can be up to 1000-fold higher compared with the planktonic phenotype. Host defence peptides (HDPs) are considered to be excellent candidates for the development of novel antibiotics. Recently, we demonstrated that a short variant of the HDP chicken cathelicidin-2, peptide FW, has potent antibacterial and lipopolysaccharide-neutralising activities. This study reports on the antibiofilm activity of peptide FW against two strains of , including a multiresistant strain. Peptide FW potently inhibited the formation of bacterial biofilms in vitro at a low concentration of 2.5μM, which is below the concentration required to kill or inhibit growth (minimal inhibitory concentration=10μM). Moreover, peptide FW also impaired existing biofilms. A 4-h challenge of pre-grown biofilms with 40μM FW reduced the metabolic activity of the wild-type strain biofilm completely and reduced that of the multiresistant strain biofilm by >50%. It is concluded that FW prevents biofilm formation and impairs mature biofilms.

Mots clés: Chicken cathelicidin-2; Biofilm; Staphylococcus epidermidis; Host defence peptide

Domaines: Microbiologie et Parasitologie

E. Margo Molhoek, Albert van Dijk, Edwin J.A. Veldhuizen, Henk P. Haagsman, Floris J. Bikker. A cathelicidin-2-derived peptide effectively impairs biofilms. International Journal of Antimicrobial Agents, 2011, 37 (5), pp.476. ⟨10.1016/j.ijantimicag.2010.12.020⟩. ⟨hal-00685789⟩